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Subsequently, a 20-g protein aliquot of each sample was mixed with an equal volume of 2 Laemmli sample buffer (Cat # 1,610,737, Bio Rad, California, USA), separated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis, and then conveyed onto a polyvinylidene difluoride membrane

Moreover, lipid ROS sensors such as Liperfluo provide real-time assessment of lipid peroxidation and ferroptosis, enabling dynamic monitoring of redox status in ischemic brain tissues (157)