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[Updated 2018 Dec 3]

Gradient elution was performed starting at a mobile phase composition of 99% solvent A and 1% solvent B, where solvent A consisted of 5 mM ammonium acetate with 0.1% acetic acid adjusted to pH 3.5, and solvent B was acetonitrile

These additionally internalized receptors are for the most part dimerized with MC3R (Schellekens et al., 2013), suggesting that dimerization with MC3R may not only lead to reduced efficiency of GHSR1a signaling, but also to reduced surface expression of the receptor