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Excessive burden of ROS, failure of clearance mechanisms and even lack of antioxidants may lead to the accumulation of ROS and to oxidative stress [40], which are associated with genetic damage and breast cancer onset [61,62,63,64,65]

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Glycolysis converts the 13 C-glucose tracer to M3-pyruvate isotopologues which are oxidized to M2-acetyl-CoA by PDC and finally condensed with oxaloacetate to M2-citrate