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For siRNA-mediated gene silencing, M0-human macrophages or BMDMs were transfected with 25 nM of Silencer pre-designed siRNA targeting human LIPE /HSL (143993 and 143994, Life Technologies), Lipe mouse pre-designed siRNA Set A (HY-RS18621, MCE), or a non-targeting control pool (Dharmacon, Lafayette, CO) using Lipofectamine LTX Transfection Reagent (Thermo Fisher Scientific)

Despite their different physiological effects, all forms of I 2 have the potential to induce or exacerbate autoantibody formation

First-time IV recipients are a unique risk group because they have no established tolerance baseline

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