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coli strains were cultured at 37C and 200 RPM in shaking incubator (IST-4075, Jeiotech, Korea)

Yuan et al., 2018), both of which are common free oxygen radicals with lipotoxicity (Mota et al., 2016)

28 November 2012

Y250 is located much farther from the disulfide bonds (Cys128-Cys206 in the hinge region between K1 and K2 domains, and Cys211-Cys288, Cys232-Cys271, and Cys260-Cys283 in the K2 domain) than the distances between Y198 and disulfide bonds, Cys149-Cys189 and Cys177-Cys201