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Having said that I used to sometimes think those injections would work better than some that had been sitting out longer, or maybe it was purely coincidence

When stomach acid production is significantly reduced, the body may have a harder time separating vitamin B12 from food sources, potentially reducing absorption over time

The eluted protein was concentrated by a size-exclusion spin column and exchanged into BRB80 buffer (80 mM HEPES, 1 mM MgCl 2 , and 1 mM EGTA, pH 6.8 by KOH) with 10% glycerol, 50 M ATP and 5 mM DTT