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HPG (0-10 mM), which can specifically inhibit carnitine palmitoyl transferase (CPT)-I activity in the mitochondria, is added in the Hepes medium before incubation of the mitochondria [1]

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PLOS ONE 12 , e0181978 (2017)

The method according to any of claims 1 to 5, wherein the derivatization reagent is dissolved in solvent to prepare the solution with concentration of 0.01100mg/ml, wherein the solvent is selected from ether, propyl ether, tetrahydrofuran, acetone, methyl ethyl ketone, acetonitrile, propionitrile, ethyl acetate, n-hexane, dichloromethane, chloroform, or their mixture of any two or more