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Investigation Areas Receptor-binding and selectivity screening (in vitro) Pathway signaling and downstream mediator assays Solubility, vehicle selection, and excipient compatibility Stability studies (temperature/light/oxidation) Method development and calibration standards Handling Requirements Use calibrated balance and sterile technique as applicable Minimize moisture exposure

When a second drug displaces a primary drug from its binding sites on plasma proteins, the unbound fraction For a low-extraction (restrictively cleared) drug, where u * CL int , The net effect is that the steady-state concentration of free drug, which is responsible for the therapeutic effect, remains largely unchanged, minimizing the risk of toxicity

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Wartolowska, K