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An imbalance in the levels ROS and RNS can induce DNA damage, pathological sarcoplasmic reticulum (SR)/Ca 2+ leakage, mitochondrial impairment, and disruption of autophagic flux, causing lipid peroxidation-associated ferroptosis and diverse types of regulated cell death (e.g., apoptosis, pyroptosis) [27]

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As this method isolates the least dense cell from a mixed population containing denser cells, it may be worthwhile considering an alternative flotation strategy