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Description

Liver slices (thickness: 200 300 m) were prepared in ice-cold KrebsHenseleit buffer, pH 7.42, supplemented with 25 mM NaHCO 3 , 25 mM D-glucose, 10 mM 2-(4-(2-Hydroxyethyl)- 1-piperazinyl)-ethanesulfonic acid (HEPES) (all from Merck, Darmstadt, Germany) and saturated with carbogen (95% O 2 , 5% CO 2 ) using a Krumdieck tissue slicer (Alabama Research and Development, Munford, AL, USA)

The reliability, validity and responsiveness of the International Restless Legs Syndrome Study Group rating scale and subscales in a clinical-trial setting