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These often subside as the body adjusts to the compound

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For DDA measurement, the same LC-gradient was measured with MS1 resolution 60,000, MS1 AGC target 3e6, MS1 max IT 45 ms, scan range 3501400 m / z , MS2 resolution 30,000, MS2 AGC target 1e5, MS2 max IT 54 ms, MS2 top6, MS2 isolation window 1.3 m / z , MS2 scan range 200-2000 m / z , MS2 NCE 28%, and MS2 dynamic exclusion 30 s