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Funding This work was supported by the CAPES (process number 88887507825/202000) and CNPq (process number 311711/20189)

Cell viability was assessed by trypan blue exclusion 51 , while crystal violet staining included 1 PBS with 20% methanol and 0.5% crystal violet for 30 min

Patschan, D

d - f , Using NET apo as an example to show the local resolution, gold-standard Fourier shell correlation (FSC) curves from the 3D refinement, and angular distribution of the particles used for the final reconstructions