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We verified both ectopic integration of F-SNIP1 variants by Sanger sequencing of their genomic DNA and F-SNIP1 expression by western blotting with SNIP1 antibody (after induction with 1 g/ml doxycycline for 24 h)

In this study, glycocalyx was observed in the entire lumen of the channels, making microchannels composed of stiff PDMS a suitable model for mechanistic studies