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This observation can be explained by the threshold effect of SDS concentration introduced by Manalastas, indicating that higher concentrations and longer exposure times do not necessarily translate into better outcomes, supported in our prior study, showing significant loss of mechanical properties, including GAG reduction, impaired collagen integrity, and altered ECM interactions after a gradual increase in SDS concentrations 36

Data was analysed by Wald test with multiple comparisons controlled with Benjamini-Hochberg correction

We treated cells with both iHAP1 [16], a PP2A agonist, and LB-100 [17], an inhibitor of PP2A, and showed that iHAP1 mediated the activation of PP2A mitigated hypoxia-induced neuronal death (Supplemental Fig

What matters most is consistent use and adequate dosing