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The expression levels of Kim-1 and NGAL were increased four to fivefold in the kidney tissue of rats exposed to the high concentration of TAA compared to the control group (Fig

S2CID 53331

Afterward, the slides were subjected to heat treatment in citrate buffer at a temperature of 95C for 10 min to enhance the process of antigen retrieval, after which they were allowed to cool for an hour and then washed three times