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The remaining fat particles were digested with 0.2% collagenase (Gibco, Billings, MT, USA) in a water bath shaker at 37 C for 30 min, and the mixture was centrifuged at 1000 rpm for 5 min to remove undigested fat

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Australian and New Zealand journal of medicine

Moreover, hormonal changes with aging such as insulin resistance, lower IGF-1 levels, and higher glucocorticoid levels can tilt the balance toward a catabolic state, meaning geriatric patients often have a blunted anabolic response even with adequate nutrients [69, 70]