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(2021) Ergothioneine mitigates cisplatin-evoked nephrotoxicity via targeting Nrf2, NF-B, and apoptotic signaling and inhibiting -glutamyl transpeptidase

We performed an immunofluorescence assay on immune cells isolated from young and aged individuals using an antibody for H2AX and a peptide nucleic acid (PNA) probe for FISH against telomeric repeats (Methods)

Excess ROS production can increase NF-kB expression in immune cells, leading to excess secretion of pro-inflammatory cytokines, including TNF-a, IL-1, IL-6, IL-8, IFN-g, iNOS, COX-2 [50]