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The full-length human ACE2 sequence without fusion fluorescent proteins was amplified from the ACE2-mKO2 fusion construct using 5-ACGACGGCGGCCGCATGTCAAGCTCTTCCTGGC-3 and 5-ACGACGGAATTCTTAAAAGGAGGTCTGAACATCATCAG-3 primers, generating a stop codon at the C-terminus, and then cloned into a lentiviral vector encoding GFP reporter separated from multiple cloning site via an internal ribosome entry site (IRES) sequence

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The growth and lignin phenotypes of C3H/APX-KD Brachypodium lines were assessed and were consistent with the phenotypes of the previously characterized and published C3H mutant in multiple alleles of A