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Cells were transfected with double-stranded siRNAs (0.5 g ml 1 ) in antibiotic- and serum-free medium for 6 h by using the X-tremeGENE siRNA transfection reagent according to the protocol provided by manufacturer (Roche Applied Sciences, Indianapolis, IN, USA) and recovered by the addition of fresh medium containing 10% FBS for 12 h

Includes insights relating to: Symptoms: High cholesterol, leg discomfort, headaches, limited physical activity, fatigue The microbiome plays a vital role in cardiovascular health through its impact on inflammation, cholesterol metabolism, and blood pressure regulation