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coli NEBExpress was first transformed with the pKD46 plasmid and then typically grown in 2 L Terrific Broth (TB) medium supplemented with 100 g/mL Amp at 30 C in the presence of 0.2 % (w/v) arabinose to induce expression of the Red genes

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On the other hand, among the 33 hub genes identified as potentially important in the hsa04010: MAPK signaling pathway of NP against SCA3, we identified CACNA1B, TNF, CACNA1H, RELA, EGFR, IKBKB, RPS6KA3, IRAK1, ERBB2, RPS6KA1, AKT1, MAPK1, IKBKG, PRKACA, MAP4K4, PRKCG, MAP4K2, HSPA8, JUN, MAP2K2, DUSP3, CHUK, PRKCA, MAPK14, VEGFA, PPM1A, TAOK1, IL1B, MAPKAPK2, MAPT, TP53, MAP3K14, and HSPA1A (Table S12)