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The top 5 precursor ions were subsequently fragmented, in a data-dependent manner, using the higher energy collisional dissociation (HCD) cell set to 30% normalized collision energy in MS2 at a resolution power of 17,500

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The released iron can be utilized for cellular processes, stored in the labile iron pool (LIP), or exported to the extracellular space via ferroportin (FPN), the only known mammalian iron exporter ( 2 + levels can trigger the Fenton reaction, in which Fe 2 + reacts with hydrogen peroxide to generate highly reactive hydroxyl radicals, leading to the accumulation of reactive oxygen species (ROS) and enhanced lipid peroxidation (Figure 1)