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Incubate 1 hour at 37 C, Aspirate and wash 3 times, Add 100L prepared Detection Reagent B

However, evening subcutaneous dosing aligns the peptide's activity with the natural cortisol decline and sleep onset window

Artlett CM, Sassi-Gaha S, Hope JL, Feghali-Bostwick CA, Katsikis PD (2017) Mir-155 is overexpressed in systemic sclerosis fibroblasts and is required for NLRP3 inflammasome-mediated collagen synthesis during fibrosis

For first timers we recommend a course of 5 infusions, the 1st and 2nd treatment being spaced 2 weeks apart, followed by the 3rd, 4th and 5th treatments once a month until the course has been finished