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Following treatment removal, all slices were maintained in serum-free medium for an additional 24 h, 1 week or 2 weeks prior to fixation and immunohistochemical analysis

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Mechanisms investigated Metabolic-pathway research converges on a small set of enzymatic and receptor targets, and these compounds map onto different ones they are not interchangeable: NNMT inhibition (5-Amino-1MQ): studied as a small-molecule inhibitor of nicotinamide N-methyltransferase, an enzyme implicated in cellular NAD/SAM metabolism