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Final reconstructions were generated using 114,673 unbinned particles without applying microtubule-specific pseudohelical symmetry (C1 reconstruction)

Since the OH group on Tyr is the phosphorylation target catalyzed by receptor tyrosine kinases (RTKs), having the steric hinderance of NO 2 on the 3-position of Tyr greatly hinders the effective phosphorylation of the 4-OH group on Tyr