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In contrast to Hoc, no clear differences between N- and C-terminal fusions of the affinity tags to Soc protein were observed (Figure 3 C and D)

RNA extraction and quantitative real-time PCR (qRT-PCR) RNA was extracted from the cochlea using an RNA Express purification kit (#RN001, Yeasen, China) according to the manufacturers instructions

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Further biochemical studies have shown increased levels of neuroketals in the SNc in post mortem tissue from Braak stages 1 and 2, supporting the notion that oxidative damage to specific lipids in the SNc occurs at very early stages of PD and prior to the appearance of LBP [83, 84]