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Briefly, 60 L of recombinant GST- SERF2 at different concentrations (250 nM and 50 nM) in binding buffer (25 mM Tris-HCl pH 7.5, 150 mM KCl or LiCl, 3 mM MgCl 2 , 500 g/mL Ultrapure BSA and SUPERase-In RNase Inhibitor) were equilibrated with 60 L of pre-washed (binding buffer) magnetic beads (Dynabeads MyOne Streptavidin T1, Invitrogen) for 30 min at 4 C

T2DM is predominantly found in adults

NADPH oxidases (NOX) are a group of proteins that transfer electrons into the cell and reduce oxygen to superoxide anion

In the present research, GPx1, as the most widely distributed and abundant in human cells (3,9,11,12), was studied