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A positive depolarized control was used, in which cells were previously incubated with 50 M CCCP for 5 min (37 C, 5% CO 2 ) and then incubated with JC-1

And in the last couple years, that includes testosterone, which is easier to get than ever before

Acknowledgments We thank all members of our laboratory for providing technical assistance

5A, B) Finally, global analysis of non-targeted metabolomics of Ms4a15 OE showed the most highly dysregulated metabolites are GP/GL lipids found in LIPID MAPS (Supplementary Fig