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However, staining procedures are time consuming, specimen-destructive and expensive

These included a cluster of phosphosites in which the basal phosphorylation was higher in WT-IR than in L973F-IR (II-B, 141 sites), a cluster in which ligand-dependent reduction of phosphorylation was greater in WT-IR than in L973F-IR (II-C, 41 sites), and a cluster in which phosphorylation of proteins was downregulated more by ligands in WT-IR than in L973F-IR (II-D, 28 sites)

Incubation of NFT with cell or tissue homogenates, microsomal preparations, or isolated mitochondria revealed one-electron reduction of NFT to yield an NFT radical anion ( NFT ) by flavoprotein-reductases such as xanthine oxidase, aldehyde dehydrogenase, thioredoxin reductase, cytochrome b 5 reductase, and cytochrome P450 reductase (CYPOR) (Miller et al

Cerretti G, Cecchin D, Denaro L, et al