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On the fifth day of differentiation, the basolateral media was removed, and the apical surface of the monolayers was washed gently three times with 100 L Dulbeccos PBS (DPBS, with calcium and magnesium

doi: 10.1016/S1471-4906(03)00142-X 3 FalAM

Aust J Crop Sci 5:347357 Rodriguez E, da Conceio SM, Azevedo R, Correia C, Moutinho-Pereira J, Ferreira de Oliveira JMP, Dias MC (2015) Photosynthesis light-independent reactions are sensitive biomarkers to monitor lead phytotoxicity in a Pb-tolerant Pisum sativum cultivar