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After completing 8 weeks on their respective diets, mice underwent cognitive studies again and were then euthanized with immediate collection of the brain for measuring outcomes

Identification of neoplastic glioma cells and differential gene expression analysis in murine tumors We identified transformed glioma cells from the murine scRNA-seq data by performing unsupervised clustering with Louvain community detection as implemented in Phenograph (Levine et al, 2015) followed by differential enrichment analysis using the binomial test to identify genes that are statistically enriched in each cluster as described previously (Shekhar et al, 2016)

You might experience temporary: Redness, itching, or soreness at the injection site Slight swelling or a small lump These reactions are usually mild and resolve on their own within a few hours