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To gain an additional layer of granularity into vulnerable neuron subpopulations in the cortex, we stained for Ctip2 to highlight neurons in layer V, including upper motor neurons, in the cortex and did not observe significant differences in neuron quantity across aging (Fig

These can be seen by putting a drop of fluid on a slide and examining it using a polarizing microscope, which takes advantage of the way uric acid crystals bend light