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Cell pellets were resuspended in 0.2 mL of buffer (150 mM NaCl, 50 mM Tris-HCl pH 8.0) for fluorescence measurement using a black 96-well plate in a SpectraMax M2e plate reader (Molecular Devices, San Jose, CA, USA) with excitation/emission wavelength pair of 488/512 nm

Some of these areas include the stomach, inner thighs, neck, buttocks and hips

While both conjugates showed cytotoxicity in isolated rat renal proximal tubular cells, cytotoxicity was reduced for OPMA but not for its S -oxide when the cells were treated with methimazole, an inhibitor of flavin-containing monooxygenase (FMO)

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