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Sci Rep 13(1):13 Cao CW et al (2022) Oral intake of chicken bone collagen peptides Anti-Skin aging in mice by regulating collagen degradation and Synthesis, inhibiting inflammation and activating lysosomes

27 Briefly, the primer pairs forward 5- GCTAGTTAAGCTTGGTACCGAGCTCGGATCCGCCACCATGATGCTTCAAC-3 and reverse 5-CTTTGTAGTCGAAGGGCCCTCTAGACTCGAGGCTCTGCAATGTTC-3 were used to construct the ATF3-WT plasmid and 5-CTTCTGGATCCCTCCCATTCTGAGCCCGGAC-3 and 5-GAATGGGAGGGATCCAGAAGATGAGAGAAACC-3 were used to construct the ATF3 phosphorylation site mutation plasmid (ATF3-Mut)

Unlike melasma, PIH has irregular borders and varies in intensity across affected areas