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The standard protocol based on clinical research and community experience: Fasted administration is important for AOD-9604 because food can interfere with its lipolytic activity

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The original genetic-validation study used antisense oligonucleotide-mediated knockdown of NNMT in adipose tissue of mice on a high-fat diet, with whole-body and adipose-tissue metabolic endpoints measured against scrambled-oligonucleotide controls [2]