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Both treatments resulted in significant reductions in BMI, hirsutism scores, fasting insulin levels, HOMA index, free testosterone levels, and menstrual irregularity compared to baseline values, demonstrating equal efficacy

the last two should be given one or two and then six months after the first

Euthanasia At the end of the experiment, rats were euthanized by an overdose of sodium pentobarbital (150 mg/kg, intraperitoneally), consistent with the AVMA Guidelines for the Euthanasia of Animals

The obtained black product was centrifuged, and then thoroughly washed with distilled water and absolute ethyl alcohol, respectively