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After the second wash, we removed the supernatant completely and resuspended the cell pellets in 1 mL of ice-cold RIPA lysis buffer containing protease or phosphatase inhibitors

Na influx has been shown to trigger glucose and lactate metabolism, further emphasizing the importance of Na in astrocytic energy regulation 103

Postabsorptive subjects completed a glycogen-depleting exercise protocol, then consumed 330ml of one of three drinks, glucose polymer solution, 8g glutamine in 330ml glucose polymer solution, or 8g glutamine in 330ml placebo, and also received a primed constant infusion of [1-13C] glucose for 2h

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