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The interactions between NPs and gastrointestinal microbiota are governed by multiple parameters, including the surface charge and physicochemical properties of both NPs and bacterial cells, the electrostatic interactions with digested food matrices, the chemical composition and bioactivity of dietary components, as well as dynamic physicochemical conditions within the gastrointestinal tract, such as pH gradients, enzymatic activity, ionic strength, and the presence of bile salts and other metabolites (Siemer et al., 2018