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Vigorous shaking introduces air bubbles and can damage the peptide through mechanical agitation

Oxygen activation and oxygen toxicity

Main body of the abstract The 70-kDa HSP (HSP70) expression is enhanced by glutamine, via the hexosamine biosynthetic pathway, which inhibits the NF-B pathway regenerating and recovering myofibers through the regulation of the early inflammatory response to muscle injury, which may be impaired by local and systemic inflammatory injury due to reduced intracellular levels of HSP70

In 4 experiments knockdown of GLUL slightly lowered the cell yield in the absence but not in the presence of glutamine ( Figure S3D )