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Catalpol alleviates Ang II-Induced renal injury through NF-B pathway and TGF-1/Smads pathway

To generate the MpkA:linker-3xHA-trpC-pyrG fusion fragment, a 2.6 Kb portion of DNA consisting of the 5-UTR region and mpkA ORF, along with a 1 Kb segment of DNA consisting of the 3-UTR flanking region were amplified with primers P19/P20 and P21/P22, respectively, from CEA17 gDNA