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plasmid #136466, Watertown, MA, United States) plasmids were performed with the transfection reagent FuGENE HD (Promega, Ref: E2311, Promega GmbH, Walldorf, Germany) at a 2:1 FuGENE HD:DNA ratio according to the manufacturer protocol

Peng HP, Jian ML, Huang ZN, Wang WJ, Deng HH, Wu WH, et al

within 10 generations)

Figure 9 In summary, through their customizable design, COF platforms not only overcome key limitations of conventional sensitizers-such as insufficient ROS generation, limited tissue penetration, and poor targeting-but also advance therapeutic paradigms from simple cell ablation to precisely inducing layered, immunogenic forms of cell death