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The doctor will order measurement of serum B12 levels, as well as other markers such as folate, homocysteine, and methylmalonic acid (MMA) levels, which are increased in B12 deficiency

In our simplified overview, we are interested in the MAP3K members named apoptosis signal-regulating kinase 1 and 2 (referred to singly as ASK1/2), the MAP2K member named MKK4, and the MAPK member named c-Jun N-terminal kinase (JNK)

Techniques for producing antibodies are well known in the art and are described, for example, in the following references: harlow and Lane "Antibodies, A Laboratory Manual", Cold Spring Harbor Laboratory Press, 1988