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Following centrifugation (20,000 x g at 4 C for 10 min), supernatants were collected, and sample protein concentrations were determined using Pierce BCA Protein Assay Kit (Thermo Scientific, 23225) with bovine serum albumin as a protein standard

For instance, post-pill amenorrhea is fairly common

It is associated with increased BDNF activity, improved cerebral blood flow, and enhanced cognitive resilience

and the staff at the Biophysics Platform, Protein Structure and Function Program from The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen for performing the human serum albumin SPR binding measurements