Although the responses on the SCAR-Q were strongly supportive of the benefits of the CO 2 laser, the remaining subjects could potentially alter this conclusion
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Total RNA was isolated using TRIzol (Invitrogen, Carlsbad, CA, USA) according to the manufacturers instructions, then 1 g of total RNA was prepared for cDNA library construction using a cDNA-PCR Sequencing Kit (SQK-PCS109, Oxford Nanopore Technologies Ltd., Oxford, UK)
Furthermore, the regulatory effect of the same inhibitor on the same deacetylase may vary with the disease context
