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Efforts to minimize animal suffering and promote welfare were paramount, and included the incorporation of anesthetics, refined handling techniques, and rigorous application of the three Rs principle (replacement, reduction, and refinement) to lessen the impact on participating animals [18]

Such factors include cytochrome C, apoptosis-inducing factor (AIF) and Smac/Diablo, which participate in the activation of intrinsic apoptotic signaling (Yang et al., 2018)

Masking was performed to remove alignment ambiguities near gaps

Psychological, immunological and physiological effects of a Laughing Qigong Program (LQP) on adolescents