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Characteristics of High Affinity Ni-Charged Resin FF 1 Dynamic binding capacity conditions: Sample: 5 mg/mL (histidine) 6 -tagged pure proteins (M r 43 000) in binding buffer (capacity at 10% Breakthrough) Column volume: 1 mL Retention time: 3min Flow rate: 0.333 mL/min Binding buffer: 20 mM sodium phosphate, 500 mM NaCl, 10 mM imidazole, pH 7.4 Elution buffer: 20 mM sodium phosphate, 500 mM NaCl, 250 mM imidazole, pH 7.4 Note: Dynamic binding capacity is protein-dependent