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Increased axonal regeneration and swellings in intraepidermal nerve fibers characterize painful phenotypes of diabetic neuropathy

Immunofluorescence staining of cultured neurons To determine the effect of lithium on neuronal morphology, neuron cultures were fixed with 4% paraformaldehyde for 30 min at room temperature, permeabilized with 0.25% Triton X-100 in phosphate buffer saline for 10 min, blocked with 5% donkey serum (Jackson Laboratories, PA) for 1 h at room temperature, and then incubated for 16 h at 4C with rabbit anti-microtubule-associated protein (MAP)2 antibody (Millipore, MA)

JanasikBReszkaEStanislawskaMJablonskaEKurasRWieczorekEet al

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