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Mayer G, Kroger M, Meier-Ewert K

In viability tests, non-viable cells absorb methylene blue staining and appear blue, while viable cells remain unstained

Complementing these findings, another study showed that supplementing high-starch calf starters (365 g of starch/kg of starter) with rumen-protected butyrate (10 g/kg butyrate/starter) during the critical two-week weaning transition increased starter intake, which may reflect a reduction in inflammation and stress during this period [16]

The expression levels of inflammatory factors (IL-1, IL-6, IL-10, TLR 4) and tight junction cytokine (ZO-1) were measured using RT-PCR