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aNSCs differentiation assay After 24 h of incubation in the proliferation medium, half of the medium was replaced with differentiation medium containing Neural Stem Cell Basal Medium, B27 without vitamin A, GlutaMAX, antibiotics (100 IU/mL penicillin and 100 g/mL streptomycin), 1 mg/mL epidermal growth factor, 1 mg/mL fibroblast growth factor 2, 1 M retinoic acid (R2625, Sigma-Aldrich), and 1 M forskolin (F6886, Sigma-Aldrich)

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Run game, just like last week, first drive, were moving down the field, got into plus territory and fumbled at the 20-yard line. Tennessee trailed 7-0 at the half, 10-7 in the third period and 17-14 in the fourth before scoring the games final ten points