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One study identified increased prodynorphin ( PDYN ) expression in the postmortem dorsolateral prefrontal cortex (dlPFC) of AUD subjects

For example, differences in the efficiency of bisulfite conversion or the depth of sequencing can cause methylation quantification to be inconsistent, which can hurt the accuracy of diagnoses ( In addition to technical standardization, the clinical applicability of methylation biomarkers in AS is limited by the restricted scale and scope of current studies, highlighting the necessity for comprehensive multicenter validation and longitudinal follow-up

Briefly, samples were mixed with Tris-EDA-HCl buffer and pyrogallol (1:15:1) and incubated at 25 C for 10 min

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