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For labelling, the fixed cells were thawed and washed for 5 min with 1 ml of 1% BSA (Sigma-Aldrich, A7906) in PBS

Risk of diabetes according to male factor infertility: a register-based cohort study

It is possible that this late change in amount and properties of microvesicles from stored RBC may be the major reason for why long stored blood units were primarily responsible for the deleterious effects in transfused patients